A B O U T    TNS Co., Ltd.

There are many companies which provide molecular diagnostic system using PCR(Polymerase Chain Reaction) system.
TNS Co., Ltd (BioTNS) is also one of these company, fountained since 2011 with goal to achieve more comfortable and more accurately detectable diagnostic system development.

Many manufactures tend to dive into Human disease diagnostic field intensively at the first time, Bio TNS expands our horizon range from disease diagnostic not only human disease for example food poisoning but also aquatic animal disease for instance Red Sea bream Iridoviral disease to species detection of bacteria, virus and animal.

All of these contents developed following to take our own line on technical training and improving method for various Real-time PCR and reverse transcription(RT) PCR techniques, based on SYBR intercalator, TaqMan probe, and PNA (peptide nucleic acid).

Since that, although we are very small and may be non-famous, absence of brand name now, We catch the many chances to gain many experiences of researching and development various contents broad ranges and co-working achievements with Public institutions including Korea National Fishery Products Quality Management Service (NFGS) and Korea National Institute of Fisheries Science, also involved many National research projects reporting meaningful business performances. In other words, It means that BioTNS has not only very strong potential in this area but also high reliability about our own performance results.

Also, BioTNS currently has TWENTY patents registration, TWENTY-SIX patents application and SIX technology transfers.

With confidence and proud of our own growing step for achieving our aims that more comfortable and more accurately detectable diagnostic system development, BioTNS looks forward to being known about our ability for developing suitable diagnostic system to large range of and satisfying various needs with our products.

Following our brand names meaning, BioTNS will always do our best with ‘Technology and Science’ in diagnostic system field. 

Digital PCR


1. Introduction of Digital PCR System


2Feature of Digital PCR System


3. General outline


4. Differentiation from current technology


5. Requirements for Next Generation Molecular Diagnostic System

Real-time PCR


  • 1. Introduction of Real-time PCR
  •  

■  Diagnosis kit based PNA probe

■  4 Channel Real-time PCR System

- Simultaneous analysis of 96 samples
- Excellent uniformity and accuracy of temperature with ±0.5℃ deviation
- 4-multiplex qPCR without the use of reference dyes
- Shortened diagnosis time with faster ramp rate being maximum of 5℃/sec

Thermal Cyclyer


1. Introduction of Smart PCR 


2. Basic Principles

The majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions – specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents – primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called Nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.

Helix OneTMLTB (Lysis Tubes with Beads)


1. Description

Helix OneTM  Lysis Tubes with Beads are designed
for rapid isolation of genomic DNA from a variety
of variety of sample sources.
Helix OneTM  Lysis Reagent & Beads make
difficult-to-lyse samples easy.
The lysis reagent & beads provide the highest
yields of nucleic acids from sample in a matter
of seconds.
Helix OneTM Lysis tubes are excellent at dispensing one uniform drop at a time. The dropping cap having breakpoint and solid cap are made of polypropylene(PP) with excellent chemical resistance. Filters can be included in the dropping caps to eliminate suspended solids.
Helix OneTM system is optimized to allow rapid and simple cell lysis followed by using advanced enzyme based technology. Helix OneTM is very simple, so you can purity DNA from a variety of target source within 5-15min.

2. DNA extraction from a varienty of sample types

* Buccal swabs, FFPE tissue, Blood, Processed foods, fungs, Fish, Plant

3. Dropping cap with filter(2 type)

 

4. Helix OneTMLTB(Lysis Tubes with Beads) : 5~15min 

DNA extraction

COVID-19 RT-PCR PNA KIT
For use only in vitro diagnostic


Product Overview


The COVID-19 RT-qPCR PNA KIT is developed with reusable fluorescence hybridization probe real-time PCR technique using Peptide Nucleic Acid (PNA), sort of artificial nucleic acid which has more higher binding ability to its complement sequence DNA than which of DNA one, to in vitro reverse transcription of SARS-CoV-2 (also known as SARS-2019-nCoV) RNA followed with cDNA amplification and detection.

The kit targets three specific genomic regions of SARS-CoV-2, those are RNA dependent RNA Polymerase (RdRP) gene , Nucleocapsid (N). These are able to detect not only individual but also in the same time. And All the Positive results from detection indicative the presence of SARS-CoV-2, expectedly.


The PNA probes for the detection of SARS-CoV-2 are labeled FAM (N gene) and HEX(RdRP) fluorescent dyes. Target amplification results are shown target dependently different colors and able to analyze which target is detected easily.


PNA, the key components of this kit development technique, is sort of artificial analog of DNA which has neutrally charged peptide backbone. It has ultimate thermo stability with high specificity to the target nucleic acid. Additionally, It has also extreme enzyme stability, in other words, enzymes such as DNA polymerase or exonuclease do not recognize to polymerization or severing partial DNA-PNA double strand. This characteristic usefully contributes to struggle for preventing false positive results and the diagnostic grey zone as well.


This Kit also include Internal Positive control, which designed on Human acidic ribosomal protein (HuPO) gene, which was reported one of the standard to very fitting for normalization of RNA expression using PCR assay, for confirming correct RNA transcription to cDNA and all the PCR reaction procedure is do on right way with reliability of performance. The PNA probe for the Internal Positive control is labeled Cy5, so it is separately able to confirm the PCR reaction such as enzyme activity and PCR equipment productivity independent to SARS-CoV-2 results.

COVID-19 RT-PCR PNA KIT
FDA EUA
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C O N T A C T

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A D D R E S S. 
6F, 19-12, Daehak-ro 76 beonan-gil,Yuseong-gu, Daejeon, Republic of Korea

T E L . 070 4256 1507                  F A X . +82 42 861 2229

E - M A I L.
biotns@biotns.com

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