About BioTNS Co., Ltd.
Growing up with strong potential in diagnostic field
BioTNS Co., Ltd (BioTNS) was established in 2011 with the aim to develop easy to use and accurate diagnostic systems.
Over the years, BioTNS has expanded its range of diagnostic technologies for the detection of such human illnesses as food poisoning, to the detection of aquatic animal diseases like red sea bream irido-virus and other human and animal bacteria and viruses.
BioTNS products are also based on various real-time PCR and reverse transcription (RT) PCR techniques, TaqMan probes, and PNA (peptide nucleic acid) identification.
In our work, we implement opportunities for research and development with public institutions such as the Korea National Fishery Products Quality Management Service (NFGS) and the Korea National Institute of Fisheries Science.
Currently, BioTNS has been involved with a national project for the development of highly-infectious disease detection systems for influenza and coronavirus diseases, as well as intestinal infections stemming from noroviruses and bacteria such as E.coli, Salmonella spp.
BioTNS is now engaged in further technical development through its new-generation PCR system “MiKro dPCR” a digital PCR which is more accurate and sensitive for quantitative analysis.
BioTNS is proud of its current achievements, and looks forward to many years of high performance in the field of Technology and Science.
■ Diagnosis kit based PNA probe
- Simultaneous analysis of 96 samples
- Excellent uniformity and accuracy of temperature with ±0.5℃ deviation
- 4-multiplex qPCR without the use of reference dyes
- Shortened diagnosis time with faster ramp rate being maximum of 5℃/sec
The majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions – specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents – primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called Nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.
Helix One™ LTB (Lysis Tube with Beads) is designed for the rapid isolation of genomic DNA from various sample sources.
Helix One™ Lysis buffer reagent and Beads make difficult samples easy to handle.
The Lysis buffer reagent and Beads provide the highest nucleic acid yields from samples in just a few seconds.
Helix One™ Lysis Tube is very useful when dispensing a uniform volume drop one at a time.
The dropping cap has a breakpoint for easy use; the solid cap is made of polypropylene (PP) with high chemical resistance. If necessary, a filter can be included in the dropping cap for eliminating suspended solid residues.
The Helix One™ system is optimized for allowing rapid and simple cell lysing for advanced enzyme-based technology.
Helix One™ is very simple and easy to use, so you can purify DNA from various target sources within 5-15mins.
*Buccal swabs, FFPE tissue, blood, processed foods, fungus, fish, plants
The COVID-19 RT-PCR PNA kit utilizes a Peptide Nucleic Acid (PNA) as a reusable fluorescence hybridization probe for real-time PCR. Peptide nucleic acids are artificially synthesized oligomers in which nucleic acid bases are attached to a neutrally charged backbone consisting of (repeating N-(2- aminoethyl)-glycine units) that form peptide bonds. PNAs exhibit stronger binding to their complementary sequences than a DNA oligomer would and cannot be degraded by exonuclease activity of enzymes such as DNA polymerase. The kit targets two specific genomic regions of SARS-CoV-2 in a multiplex reaction: the RNA-dependent RNA Polymerase (RdRP) gene and the Nucleocapsid (N) gene. The PNA probes for the detection of SARS-CoV-2 are labeled with FAM (N gene) and HEX (RdRP) fluorescent dyes. Individual target amplification results are identified with different colors and thus able to be independently analyzed. This kit also includes an internal control which amplifies the Human acidic ribosomal protein (HuPO) gene. Successful amplification of HuPO confirms correct RNA transcription to cDNA and subsequent PCR amplification. The PNA probe for the HuPO gene is labeled with a Cy5 fluorophore.